{"ID":2846529,"CreatedAt":"2026-06-01T04:54:23.091178241Z","UpdatedAt":"2026-06-01T04:54:23.091178241Z","DeletedAt":null,"paper_url":"https://arxiv.org/abs/2511.01277","arxiv_id":"2511.01277","title":"Identification of Capture Phases in Nanopore Protein Sequencing Data Using a Deep Learning Model","abstract":"Nanopore protein sequencing produces long, noisy ionic current traces in which key molecular phases, such as protein capture and translocation, are embedded. Capture phases mark the successful entry of a protein into the pore and serve as both a checkpoint and a signal that a channel merits further analysis. However, manual identification of capture phases is time-intensive, often requiring several days for expert reviewers to annotate the data due to the need for domain-specific interpretation of complex signal patterns. To address this, a lightweight one-dimensional convolutional neural network (1D CNN) was developed and trained to detect capture phases in down-sampled signal windows. Evaluated against CNN-LSTM (Long Short-Term Memory) hybrids, histogram-based classifiers, and other CNN variants using run-level data splits, our best model, CaptureNet-Deep, achieved an F1 score of 0.94 and precision of 93.39% on held-out test data. The model supports low-latency inference and is integrated into a dashboard for Oxford Nanopore experiments, reducing the total analysis time from several days to under thirty minutes. These results show that efficient, real-time capture detection is possible using simple, interpretable architectures and suggest a broader role for lightweight ML models in sequencing workflows.","short_abstract":"Nanopore protein sequencing produces long, noisy ionic current traces in which key molecular phases, such as protein capture and translocation, are embedded. Capture phases mark the successful entry of a protein into the pore and serve as both a checkpoint and a signal that a channel merits further analysis. However, m...","url_abs":"https://arxiv.org/abs/2511.01277","url_pdf":"https://arxiv.org/pdf/2511.01277v1","authors":"[\"Annabelle Martin\",\"Daphne Kontogiorgos-Heintz\",\"Jeff Nivala\"]","published":"2025-11-03T06:51:53Z","proceeding":"cs.LG","tasks":"[\"cs.LG\",\"q-bio.QM\"]","methods":"[\"Convolutional Neural Network\"]","has_code":false}